The Differences between DNA Polymerase 1 Vs 3
Science is being very advance by which the world is progressing. Most of the people are confused about what is the difference between DNA polymerase 1 vs 3? In this article, I will try to explain the difference between DNA polymerase 1 vs. 3.DNA stands for Deoxyribonucleic Acid.
Who discovered DNA?
Many people believe that American biologist James Watson and English physicist Francis Crick discovered DNA in the 1950s. In reality, this is not the case. Rather, DNA was first identified in the late 1860s by Swiss chemist Friedrich Miesche.
DNA polymerase was discovered by Arthur Kornberg and the DNA polymerase 3 was discovered by Thomas Kornberg and Malcolm.
Varieties of DNA polymerases but all are based on the different function which they have to perform. DNA polymerase 1 is also called “pol1”. It is essential for DNA replication. Here used replication before going ahead you must know what is this?
Replication: DNA produces carbon copies through replication. It allows DNA transfer genetic information from old cells to new cells (from one generation to the next generation).
DNA polymerase 3 is also called “Holoenzyme”. It is the most important component of the replisome.
DNA joining is don-e by another enzyme called ligase.
DNA Polymerase 1 is a function that helps in DNA replication. It is used for molecular biology research. RNA primmer is filled in the lagging strand of the DNA. Then, DNA polymerase fills in the nucleotides after removing the RNA primer. Which are the essential for the formation of the DNA in the direction 5 to 3? It not only helps in that but also helps in the proof for seeing provided that any mistake is done while replication and matching base pairs.
The fact which we must remember is that Polymerase 1 only adds the nucleotides but it does not joint them. You must remember that DNA joining is done by another enzyme called ligase. This forms Continuous Strands of DNA. The important function of the polymerase 1 is the DNA labeling by nick translation and second stand synthesis of cDNA. DNA polymerase 1 also catalyzes 5’ to 3’ synthesis of DNA. DNA polymerase 1 reads the shape and polarity of the incoming dNTP. DNA polymerase 1 has 3 activities like polymerase, 3’ to 5’ exonuclease and 5’ to 3’ exonuclease. DNA polymerase 1 is a template-dependent DNA polymerase.
DNA polymerases are specially designed enzymes. They assemble tiny buildings blocks of DNA. This helps them to collect information of DNA Molecules.
They are called nucleotides. DNA polymerase distributed into two identical DNAs. The dividing process of DNA is called DNA replication. DNA polymerase works as a catalyst in DNA replication and it is very important. Two new strains are created by DNA strands which match the present DNA. In this way, the genetic information is forward into the daughter cells and transforms one generation to another generation.
The Pol 3 synergist place has firmly bound subunits called alpha, epsilon, and theta. The alpha subunit is answerable for the DNA polymerase movement, the epsilon subunit has editing exonuclease action and the theta subunit is the littlest of all and aides in improving the editing properties of epsilon. A Replisome is situated at the replication fork. DNA polymerase 3 is a part of the replisome and consequently helps in replication.
|DNA Polymerase 1||DNA Polymerase 3|
|DNA polymerase 1 is a DNA polymerase encoded by the pola gene and is involved in the prokaryotic DNA replication.||DNA polymerase 3 is the main enzyme that aids prokaryotic DNA replication.|
|Removes the RNA primer.||Requires an RNA primer to synthesize The DNA.|
|Adds nucleotides to the growing polynucleotide chain.||The key enzyme for synthesizing DNA in prokaryotes.|
|Removes the RNA primer from 5 to 3 directions.||Adds deoxyribonucleic acids to the DNA.|
|Has both 3 to 5 exonuclease activity and 5 to 3 exonuclease activity.||Only has 3 to 5 exonuclease activity.|
|Encoded by polyA gene.||Encoded by DNA DNAQ and hole genes.|
|First discovered by Arthurn Kornberg in 1956.||First discovered by Thomas Kornberg and Malcolm Gefer in 1970.|
|Belongs to the DNA polymerase family A.||Belongs to the DNA polymerase family c.|
|Only acts on the lagging strand.||Acts on both leading and lagging strands of the replication fork.|
|Can add 10 to two nucleotides per second.||Can add around 1000 nucleotides per second.|
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